EMB contains lactose and sucrose sugars, but it is the lactose that is the key to the medium. Lactose-fermenting bacteria (E. coli and other coliforms) produce acid from lactose use, and the combination of the dyes (which serve as pH indicators in this medium) produces color variations in the colonies because of the acidity. Strong acidity produces a deep purple colony with a green metallic sheen, whereas less acidity may produce a brown-pink coloration of colony. Nonlactose fermenters appear as translucent or pink.
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Overview
- Condition: New
Features:
- EMB agar is also used to differentiate the organisms in the colon-typhoid-dysentery group: Escherichia coli colonies grow with a metallic sheen with a dark center, Aerobacter aerogenes colonies have a brown center and non-lactose-fermenting, gram-negative bacteria appear pink.
