MacConkey agar is used for the isolation of gram-negative enteric bacteria and the differentiation of lactose fermenting from lactose non- fermenting gram-negative bacteria. It has also become common to use the media to differentiate bacteria by their abilities to ferment sugars other than lactose.
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Overview
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Features:
- Key components of the MacConkey medium include crystal violet dye, bile salts, lactose, and neutral red (pH indicator). Crystal violet dye and bile salts halt the growth of gram-positive bacteria. This allows only gram-negative species to form colonies on MAC agar.
